胆囊癌组织端粒酶活性的检测及其意义

Telomerase activity detection in gallbladder carcinoma and its significance

摘要: 目的: 探讨胆囊癌组织中端粒酶活性表达及意义。方法: 采用TRAP-PAGE-银染法检测22例胆囊癌及28例慢性胆囊炎新鲜组织中端粒酶活性。结果: 28例慢性胆囊炎组织中,无端粒酶活性阳性表达;22例胆囊癌组织中,端粒酶活性表达阳性者18例,阳性率为81.8%,两者差异有显著性(P<0.005)。端粒酶活性表达与胆囊癌的分化程度及淋巴结转移均无关(P>0.05)。结论: 检测端粒酶活性对胆囊癌诊断有一定参考价值。

Abstract: Objective: To investigate the significance of the expression of telomerase in chronic cholecystitis and gallbladder carcinoma.Methods: The telomerase activity in 22 cases of gallbladder carcinoma and 28 cases of chronic cholecystitis was measured.Results: The positive expression rate of telomerase in the cases with gallbladder carcinoma was 81.8%,but no positive reaction was detected in the cases with chronic cholecystitis.The difference was significant(P<0.005).Telomerase activity was not obviously correlated with the differentiation and lymphatic metastasis of gallbladder carcinoma(P>0.05).Conclusions: Detecting the activity of telomerase is of reference value for the diagnosis of gallbladder carcinoma.