Abstract: Objective: To investigate the clinical value of laparoscopy-assisted vaginoplasty with free-lamellar peritoneum for patients with congenital absence of vagina.Methods: The clinical data of 18 cases with congenital absence of vagina received laparoscopy-assisted vaginoplasty with free-lamellar peritoneum were reviewed,and the operation outcome was analyzed.Results: The operation was successful in all the 18 cases,and no complications occurred.All the cases were followed up for 3 to 12 months.The artificial vaginas were found to be in good growth,and the vaginal wall was pale red,soft and moist with good elasticity.All the cases had a normal vaginal appearance and satisfactory sexual life.Conclusions: Laparoscopy-assisted vaginoplasty with free-lamellar peritoneum is safe and has the advantages of minimal invasion,rapid recovery and good therapeutic efficacy.It is an ideal modus operandi for patients with congenital absence of vagina.