Abstract: Objective: To explore the relativity of in vitro and in vivo examinations of the allergen was performed in etiology of the chronic urticaria.Methods: In vivo examination of the allergen(the skin test) in 1 335 patients of chronic urticaria.In vitro examination of the allergen(serum specific IgE) was performed in 254 patients at the same time.Results: The positive rate of skin test was 78.8%(1 052/1 335).The most common allergen in inhalation were the dermatophagoides pteronyssinus,dust mites,polyvalent fungs I and pollen of summer and autumn.The most common allergen of food group are shrimp,sesame,tomato,ginger.The positive rate of serum specific IgE 65.7%(167/254).The most common allergen in inhalation were the dermatophagoides pteronyssinus,penicillium notatum,aspergillus fumigatus,and mugwort pollen.The most common allergen of food group were the shrimp,tomato.Conclusions: The most common allergen with chronic urticaria in Huaibei are dermatophagoides pteronyssinus,penicillium notatum,aspergillus fumigatus,and mugwort pollen,shrimp,tomato.The match ratio of in vitro and in vivo examinations of the allergen is high.