脑胶质瘤组织中血管内皮生长因子的表达意义

Expression of vascular endothelial growth factor in human gliomas

摘要: 目的: 探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)在脑胶质瘤组织中的表达及其意义。方法: 采用免疫组化和核酸原位杂交的方法,检测68例脑胶质瘤和10例正常脑组织标本中VEGF的表达。结果: VEGF蛋白和mRNA在胶质瘤中的阳性表达率随着胶质瘤病理分级的增加而增加,差异均有显著性(P<0.01),而正常脑组织不表达。结论: VEGF高表达与胶质瘤的恶性进展有着密切关系。VEGF可能通过参与诱发胶质瘤血管的生成,对胶质瘤的演进过程起促进作用。

Abstract: Objective: To explore the expression of vascular endothelial growth factor(VEGF) and its relationship with progression of human brain glioma.Methods: VEGF protein was detected by immunohistochemistry in 60 cases of glioma and 10 cases of normal brain tissues.Nucleic acid hybridization in situ was used to examine the expression of VEGF mRNA in the tissues.Results: The positive expression of VEGF protein and VEGF mRNA increased significantly with the pathological grades of glioma(P<0.01).There was no expression in the normal human brain.Conclusions: The high expression of VEGF is obviously related to the progression of human glioma.VEGF may contribute to the progression of human glioma by playing a part in the formation of angiogenesis.