万古霉素耐药金黄色葡萄球菌耐药机制探讨

The mechanism exploration of vancomycin resistant Staphylococcus aureus

摘要: 目的: 探讨金黄色葡萄球菌(金葡菌)对万古霉素的耐药机制。方法: 应用万古霉素体外诱导异质性万古霉素耐药金葡菌株,PCR检测金葡菌特异性nuc基因和万古霉素耐药vanA基因。结果: 万古霉素耐药金葡菌含有nuc基因,但无vanA基因。结论: 万古霉素耐药金葡菌不含有常见的万古霉素耐药基因,细胞壁增厚可能是金葡菌对万古霉素耐药的主要机制之一。

Abstract: Objective: To study the drug resistance mechanism of Staphylococcus aureus (aureaus) to vancomycin. Methods: Vancomycin was applied in vitro to induce vancomycin resistant heterogeneity aureaus strains; then nuc gene(aureaus specificity gene) and vanA gene(vancomycin resistance gene) were detected by PCR. Results: Vancomycin resistant aureaus contained nuc gene,but no vanA gene. Conclusions: Vancomycin resistant aureaus contains no common vancomycin resistant gene,cell wall thickening is maybe one of the main mechanisms of aureaus to vancomycin resistance.