Abstract: Objective: To investigate the effects of compound Hugan decoction on conA-induced liver injury in mice. Methods: BALb/c mice were randomly divided into six groups,10 in each,which included normal,liver injury,biphenyldimethylesterate treatment 1.5 mg·kg^-1·d^-1 and compound Hugan decoction treatment(7 g·kg^-1·d^-1,14 g·kg^-1·d^-1,28 g·kg^-1·d^-1) groups. The animal model of liver injury was established by intravenous injection of ConA (12.5 mg/kg) in BALb/c mice. The serum level of alanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP), γ-glutamyltransferase(GGT), and hyaluronidase(HA) were assayed by spectrophotometer. Results: Compound Hugan decoction significantly decreased the concentration of serum transaminases(ALT,AST,ALP,GGT) and it showed a dose-dependent manner between the range of 7-28 g·kg^-1·d^-1. Conclusions: Compound Hugan decoction performs protective effect against ConA-induced liver injury in mice. Such protective effects might be related to the increase of antioxidant enzyme activity and the promotion of free radicals elimination.