Abstract: Objective:To comprehend the distribution of common pathogens and drug resistance in clinical infection,and provide the basis for rational use of antimicrobial drugs to clinicians. Methods:The statistical distribution of 602 strains of bacteria isolated from clinical samples in 2009 and the bacterial resistance rates of common infection were analyzed. Results:There were 134 strains of Gram-positive bacteria(22.26%),468 strains of Gram-negative bacteria(77.74%) in 602 strains of isolated bacteria.In Gram-positive bacteria infection,Staphylococcus epidermidis infection was more common(11.79%),followed by other streptococci(5.81%);while E.coli infection(29.57%) was at the top in Gram-negative bacteria infection,followed by Enterobacter(13.29%) and Klebsiella(12.46%).Drug susceptibility analysis revealed that Staphylococcus epidermidis was sensitive to novobiocin and ampicillin 100.00% and resistant to aztreonam,azithromycin and penicillin,the resistance rates were respectively 96.00%,96.00% and 92.42%.E.coli was sensitive to nitrofurantoin and 100.00% resistant to penicillin,rifampin and lincomycin. Conclusions:The drug resistant rates of penicillin,lincomycin and azithromycin were very high,the drug resistant rates of Gram-positive bacteria to ampicillin,vancomycin,novobiocin are low;the Gram-negative bacteria is the most sensitive to cefoperazone/sulbactam,tazobactam,piperacillin/tazobactam.