大鼠肝再生过程中增殖细胞核抗原的表达

Expression of proliferating cell nuclear antigen during liver regeneration process after partial-hepatectomy in rats

摘要: 目的: 探讨大鼠肝再生过程中肝细胞增殖细胞核抗原(PCNA)表达的变化。方法: 建立肝切除动物模型,用免疫组织化学法、图像分析仪检测肝细胞PCNA的表达。结果: PCNA强阳性肝细胞数及其总灰度值在肝大部切除后24 h明显增高,肝细胞阳性表达率在48 h达到峰值,至120 h趋于正常水平。结论: PCNA强阳性肝细胞呈规律性变化,提示残余肝可再生。

Abstract: Objective: To observe the expression of proliferating cell nuclear antigen(PCNA) during liver regeneration process after partial-hepatectomy in rats.Methods: ABC immunohistochemistry methods and anti-PCNA monoclonal antibody were used to reveal the expression of PCNA in the rat liver slice.Results: The number of PCNA positive cells and the grey degree numerical value increased remarkably 24 h after hepatectomy,and the expression rate of heptocytes increased to peak valuer 48 h after hepatectomy. Conclusions: The PCNA strong positive cells show regular change afterhepatectomy,which suggests that the remained liver cells can regenerate.