Abstract: Objective: To analyze the detection and distribution of vaginitis related pathogens of the outpatients in the gynecology department of our hospital. Methods: Four thousand four hundred and fifty-six vaginal discharge samples were detected of the fungus， bacteria， trichomonas and cleanness degree by vaginitis penta-pathogen detection kit. The detection rates in different age groups and the cleanness grades were compared. Results: The detection rate of various pathogens in patients with Ⅲ － Ⅳ grades of vaginal cleanness was higher than that in patients with Ⅰ － Ⅱ grades of vaginal cleanness ( P ＜ 0. 01) . The detection rate of fungus，bacteria and trichomonas in the age groups of 26 － 35 and 36 － 45 was higher than that in the age groups of 25， 46 － 55 and ＞ 55( P ＜ 0. 05) . Conclusions: The most common pathogens in the vaginitis of the outpatients in the department of gynecology were fungus，bacteria and trichmonas. Vaginal discharge with normal cleanness should also be detected of the pathogens.