Abstract: Objective: To carry out targeted training by surveying the apprentice nurses' mastery of hospital infection knowledge. Methods: A total of 180 apprentice nurses were investigated about the hospital infection knowledge by a questionnaire before and after the training. Results: The score increased significantly after training compared to that before， the difference was statistically significant ( P ＜ 0. 01) . Conclusions: The apprentice nurses' knowledge about hospital infection is quite deficient before training. Pro-job training can significantly improve their mastery of hospital infection knowledge，which is essential in prevention and control of hospital infections.