Abstract: Objective: To analyze the value of fine-needle aspiration biopsy(FNAB)in diagnosis of thyroid nodus.Methods: A total of 94 cases with thyroid nodus were analyzed between FNAB and histopathology.Results: The results of FNAB analysis were shown: 81 cases of benign lesion, including 60 cases of nontumorous changes and 21 cases of adenoma(the results of histopathology were that 2 cases of papillary adenoma,and 19 cases of follicular adenoma); 9 cases of malignant changes including 3 cases of papillary adenocarcinoma,2 cases of undifferentiated carcinoma and 4 cases of adenocarcinoma(the results of histopathology were that 4 cases of papillary carcinoma,1 case of medullary carcinoma,3 cases of undifferentiated carcinoma and 1 case of follicular adenocarcinoma); and other 4 cases were unsuccessfully diagnosed by FNAB which reported that the glandular cells had obvious atypia,suggesting to do further examination(the results of histopathology were that 3 cases of follicular adenocarcinoma and 1 case of follicular adenoma). Conclusions: FNAB plays an important role in identifing benign and malignant thyroid nodules, and diagnosing hashimoto thyroiditis and subacute thyroiditis definitely.The use of FNAB could improve the efficiency of definite diagnosis in common thyroid disease.