Abstract: Objective: To investigate the expressions of focal adhension kinase( FAK) and phosphatase and tensin homology deleted on chromosome ten( PTEN) in human glioma and their relationships with the occurance,invasive growth and clinical pathological features of tumor. Methods: The expression levels of FAK and PTEN in 6 cases of normal brain tissue and 54 cases of human glioma samples were detected by EliVision immunohistochemical method. Results: The total positive rate of FAK expression in glioma was 66. 67% including stageⅠto Ⅱ( 35. 00%),stage Ⅲ( 81. 25%) and stage Ⅳ( 88. 89%),the positive rates of FAK expression in stage Ⅰto Ⅱwere lower than that in stage Ⅲ and Ⅳ( P 0. 01) and its expressions in grade Ⅲ and Ⅳ were not statistical significance( P 0. 05). The total positive rate of PTEN expression in glioma was 46. 30% including stageⅠto Ⅱ( 75. 00%),stage Ⅲ( 31. 25%) and stage Ⅳ( 27. 78%),the positive rates of FAK expression in stageⅠto Ⅱwere higher than that in stage Ⅲ and Ⅳ( P 0. 01) and its expressions in stage Ⅲ and Ⅳ were not statistical significance( P 0. 05). The FAK and PTEN expression in glioma was negative correlation( P 0. 01). Conclusions: The positive rates of FAK and PTEN expression increase and decrease with the increase of glioma pathological grade,respectively. These tumor marker expressions can help determine the pathological stage and prognosis of glioma,and further guide clinical treatment.