Abstract: Objective: To investigate the value of colposcope biopsy combined with loop electrosurgical excision procedure( LEEP) in the diagnosis and treatment of cervical high-grade intraepithelial neoplasia( CIN). Methods: One hundred and three patients with grade 2 and 3 CIN diagnosed by colposcope biopsy were treated with LEEP. The postoperative pathological findings were compared with the preoperative pathologic results. Results: The operations of 103 cases were successful. The postoperative pathology of 80 cases was still CIN 2 and 3,the coincidence rate of which was 77. 67%. The postoperative pathological levels of 21 cases( 20. 39%)( including chronic cervicitis in 16 cases and CIN 1 in 5 cases) declined. The postoperative pathological levels of 2 cases( 1. 94%)( cervical invasive carcinoma) increased. Conclusions: The colposcope cannot obtain the tissue of cervical canal lesions,which has certain limitation in the diagnosis of cervical lesions. LEEP resection can arrive at the enough scope and depth of lesion. The colposcope combined with LEEP can improve the diagnosis and treatment of grade 2 and 3 CIN.