Abstract: Objective: To investigate the apoptosis of macrophage induced by different flora Candida albicans,and provide a new insight for the prevention and treatment of disease. Methods: The THP-1 cells were induced to differentiate into macrophage by phorbol myristate acetate. The macrophage was infected by yeast form,hyphal form of Candida albicans and Candida albicans pre-treated with prepstatin( PEP) for 1 hour,2,4 and 8 hours,the blank control group was also established. The apoptosis rate of macrophage was detected by flow cytometer. Results: Compared with the blank control group,the apoptosis rates of macrophage infected by the yeast form,hyphal form of Candida albicans and Candida albicans pre-treated by PEP for 1 hour,2,4 and 8 hours significantly increased( P 0. 05 to P 0. 01),the apoptosis rate of macrophage increased with the extension of infection time( P 0. 01). Compared with macrophage infected by Candida albicans pre-treated by PEP,the apoptosis rates of macrophage infected by the yeast form and hyphal form of Candida albicans significantly increased in different time-point( P 0. 01). The apoptosis rate of macrophage infected by hyphal form of Candida albicans was significantly higher than that in macrophage infected by yeast form( P 0. 01). Conclusions: Candida albicans can induce the apoptosis of macrophage,the effect of which is related to the form of Candida albicans and secreted aspartic proteinase.