Abstract: Objective:To survey the serotype and antibiotics resistance of enteropathogenic Escherichia coli(E.coli) in children with diarrhea in Nanjing area. Methods:Five thousand four hundred and nine cases of feces samples were cultured,isolated E.coli strains were indentified by serotyping,and the antibiotic susceptibility was determined by disk diffusion test. Results:Six hundred and thirtythree strains of E.coli were isolated(11.70%) and 12 serotypes were detected. The incidence of ESBLs was 82.15% in the isolated strains. Conclusions:E.coli is an important pathogen leading to infant diarrhea,and highly resistant to many antibiotics; we should identify E.coli and select correct antibiotics according to the results of susceptibility test.