三氧化二砷对鼠恶性黑素瘤B<sub>16</sub>细胞凋亡的影响

三氧化二砷对鼠恶性黑素瘤B₁₆细胞凋亡的影响

摘要: 目的: 探讨三氧化二砷(As₂O₃)对鼠恶性黑素瘤B₁₆细胞诱导凋亡的影响。方法: 采用流式细胞术(FCM)检测不同浓度的As₂O₃诱导B₁₆细胞的早期凋亡率、晚期凋亡率和总凋亡率。结果: As₂O₃浓度分别为5μmol/L、10μmol/L、20μmol/L时,B₁₆细胞的早期凋亡率分别为7.18%、21.1%、3.59%,晚期凋亡率分别为2.63%、5.35%、6.34%,总凋亡率分别为9.81%、26.45%、9.93%。结论: B₁₆细胞的早期凋亡率和晚期凋亡率对As₂O₃有浓度依赖关系,高浓度的As₂O₃使B₁₆细胞出现坏死。

Abstract: Objective: To study the effect of arsenic trioxide on apoptosis of melanoma B₁₆ cells.Methods: Early apoptosis rate,late apoptosis rate and total apoptosis rate in melanoma B₁₆ cells were analysed by flow cytometry.Results: At the doses of arsenic trioxide were 5 μmol/L,10 μmol/L,or 20 μmol/L,the early apoptosis rates in melanoma B₁₆ cells were 7.18%,21.1%,and 3.59%,respectively;the late apoptosis rates were 2.63%,5.35%,and 6.34%,respectively;the total apoptosis rates were 9.81%,26.45%,and 9.93%,respectively.Conclusions: The early apoptosis rate and the late apoptosis rate in melanoma B₁₆ cells have dose-dependent relationship with arsenic trioxide.