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    俞小忠, 王勇, 陈芝河, 项小春. 人血清中青霉素抗体的酶联免疫吸附测定[J]. 蚌埠医科大学学报, 2007, 32(5): 517-518.
    引用本文: 俞小忠, 王勇, 陈芝河, 项小春. 人血清中青霉素抗体的酶联免疫吸附测定[J]. 蚌埠医科大学学报, 2007, 32(5): 517-518.
    shu
    YU Xiao-zhang, WANG Yong, CHEN Zhi-he, XIANG Xiao-chun. Detection of penicillin antibodies in human serum by enzyme-linked immunosorbent assay[J]. Journal of Bengbu Medical University, 2007, 32(5): 517-518.
    Citation: YU Xiao-zhang, WANG Yong, CHEN Zhi-he, XIANG Xiao-chun. Detection of penicillin antibodies in human serum by enzyme-linked immunosorbent assay[J]. Journal of Bengbu Medical University, 2007, 32(5): 517-518.
    shu

    人血清中青霉素抗体的酶联免疫吸附测定

    Detection of penicillin antibodies in human serum by enzyme-linked immunosorbent assay

      摘要
    • 摘要: 目的: 建立检测青霉素抗体的ELISA法,并探讨其临床意义。方法: 以氨苄西林用SPDP法与兔血清白蛋白交联,用此交联物为包被抗原,HRP-抗人IgG为酶标抗体,建立检测青霉素抗体的ELISA法,并对部分临床标本进行青霉素抗体检测。结果: 建立的方法其批内、批间变异系数分别为6.8%、8.4%。氨苄西林-兔白蛋白交联物免疫家兔获得兔抗氨苄西林的抗体,以此抗体作阻断试验,抑制HRP-抗人IgG的显色。临床标本检测结果表明,不同疾病患者青霉素抗体的阳性率不同。结论: ELISA可用于临床对青霉素抗体的检测,具有较好的特异性、敏感性和重复性。

       

      Abstract: Objective: To establish an enzyme-linked immunosorbent assay (ELISA) for detection of the antibody of penicillin in human serum. Methods: The ELISA to detect the penicillin antibody was established by adding sera into the polystyrene micro-titer plates coated with penicillin-conjugated rabbit serum albumin and HRP-conjugated goat anti-human IgG. The Penicillin antibody in some clinical samples was detected with this method. Results: The within and between assay CV were 6.8% and 8.4% respectively. The rabbit anti-penicillin antibody, which was obtained from penicillin-conjugated rabbit serum albumin, could control the HRP-anti human IgG reaction. The positive rate of penicillin antibody in patients with different diseases was significantly different. Conclusions: ELISA is more sensitive and specific in clinically detecting and antibody of penicillin.

       

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