SPRR1A基因真核荧光表达载体的构建

Construction for fluorescent eukaryotic cell expression vector of small proline-rich repeat protein 1A gene

摘要: 目的:构建可在真核细胞中表达SPRR1A(small proline-rich repeat protein1A)的荧光表达载体。方法:应用PCR技术扩增出编码SPRR1A的全长基因,将SPRR1A DNA克隆到pEGFP-N1质粒,通过抗性基因筛选阳性克隆,经酶切和测序鉴定。结果:酶切和DNA序列鉴定均证实插入片段与GenBank提供的序列(L05187)一致。结论:成功构建了SPRR1A真核荧光表达载体。

Abstract: Objective: To construct fluorescent eukaryotic expression vector of small proline-rich repeat protein 1A(SPRR1A) gene.Methods: The coding sequence of SPRR1A was amplified by polymerase chain reaction,the SPRR1A gene was cloned into plasmid pEGFP-N1,and the recombinant vector was selected and identified by restriction enzyme analysis and nucleotide sequence determination.Results: Correct construction of pEGFP-N1-SPRR1A was identified by methods of restriction enzyme analysis and nucleotide sequence determination.Conclusions: Eukaryotic expression plasmid pEGFP-N1-SPRR1A has been successfully constructed.