Effect of GPD2 gene knockdown by shRNA on the biological function of lung cancer cells H1299

ObjectiveTo investigate the effects of silencing the expression of glycerol-3-phosphate dehydrogenase 2 (GPD2) gene on the biological function (proliferation, apoptosis and invasion) and behavior of lung cancer cells.

Methods The RNA interference lentiviral vector containing GPD2 gene (shGPD2) and corresponding control vector (shCtrl) were constructed, and used to infect the H1299 cells.The expression levels of GPD2 gene mRNA and protein in cancer cells were detected using qPCR and Western blotting, respectively.The knockdown efficiency of GPD2 gene was verified.The proliferation, apoptosis and invasion of cells were detected using CCK8 method, flow cytometry and Transwell assay, respectively.

ResultsThe lentiviral vector shGPD2 was successfully constructed, and infected H1299 cells.The mRNA and protein levels decreased, and the knockdown efficiency was 73.5% (P < 0.05).After the GPD2 gene expression decreased, the proliferation, apoptosis and invasion ability of H1299 cells significantly slowed down, increased and reduced, respectively (P < 0.01).

ConclusionThe GPD2 gene knockdown can effectively inhibit the proliferation of lung cancer cells H1299.