ObjectiveTo detect the activity levels of serum mitochondrial acetaldehyde dehydrogenase 2 (ALDH2) and nucleotide-binding oligomerization domain-like receptors protein 3 (NLRP3), analyze the correlations between ALDH2, NLRP3 and different courses of coronary heart disease (CHD), and explore the change rule of both in the occurrence and development of CHD.
MethodsA total of 170 chest pain patients detected by coronary angiography were investigated, and 133 CHD patients and 37 normal patients (control group) were identified.One hundred and thirty-three CHD patients were divided into the mild stenosis group (19 cases with stenosis deree of < 50%, is-CHD group), moderate stenosis group (41 cases degree of stenosis with 50% to 75%, ms-CHD group) and severe stenosis group (73 cases with stenosis degree of ≥75%, ss-CHD group) according to the severity of coronary artery stenosis.The activity levels of ALDH2 and NLRP3 in all cases were detected using enzyme-linked immunosorbent assay.The differences of the activity levels of ALDH2 and NLRP3 in CHD patients with different degrees of disease were analyzed, the correlations between activity levels of ALDH2, NLRP3 and occurrence, development of CHD were disscussed.The serological influencing factors of ALDH2 and NLRP3 were analyzed using the Pearson correlation analysis.
ResultsThe serum level of creatinine in CHD group was higher than that in control group and ms-CHD group (P < 0.05), the high-density lipoprotein level in ss-CHD group was significantly lower than that in control group (P < 0.01), while the lymphocyte/monocytes in ms-CHD group and ss-CHD group were lower than that in the is-CHD group (P < 0.05).The serum levels of ALDH2 in ms-CHD and ss-CHD groups were lower than that in control group, which gradually decreased with the aggravation of the severity of coronary artery stenosis (P < 0.05-P < 0.01), while the serum level of NLRP3 gradually increased with the aggravation of the degree of coronary artery disease (P < 0.01), and there was a positive correlation between both (P < 0.05).With the increasing of the number of CHD lesions, the level of ALDH2 gradually decreased (P < 0.01), while the level of NLRP3 gradually increased (P < 0.05), and the change of NLRP3 was significant.The serum level of ALDH2 was negatively correlated with Gensini score (r=-0.247, P < 0.01), while the NLRP3 level was positively correlated with Gensini score (r=0.477, P < 0.01).The serum level of ALDH2 was negatively correlated with LDL (r=-0.220, P < 0.05) and the serum level of NLRP3 was negatively correlated with ALDH2 (r=-0.201, P < 0.05).
ConclusionWith the aggravation of coronary artery disease, the serum level of ALDH2 gradually decreases, the serum level of NLRP3 gradually increases, and there was a certain correlation between them.
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