Relationship between the serum levels of thrombospondin, matrix metalloproteinase and cerebral hematoma and edema in the acute phase of cerebral hemorrhage

ObjectiveTo study the relationship between the serum levels of matrix metalloproteinase (MMP), thrombospondin (TSP) and cerebral edema and hematoma in acute phase of cerebral hemorrhage.

MethodsSixty-two patients with acute hypertensive cerebral hemorrhage and 50 normal people were divided into the observation group and control group, respectively.The serum in observation group on the 1st, 2nd, 3rd, 5th and 7th day and seventh day of onset and control group at physical examination were collected.The levels of MMP 2, MMP9, TSP1 and TSP2 in two groups were detected using enzyme-linked immunosorbent assay (ELISA).The CT scan of brain in two groups was simultaneously performed to measure the volume of cerebral hematoma and edema.The correlations of the serum levels of MMP 2, MMP9, TSP1 and TSP2 with volume of cerebral hematoma and cerebral edema were analyzed.

ResultsThe serum levels of MMP 2, MMP9 and TSP1 in observation group at different time-points were significantly higher than those in control group (P < 0.01).The levels of MMP2, MMP9 and TSP1 in observation group on the 3rd, 5th and 7th days were significantly higher than that on the 1st day (P < 0.01), reached a peak on the 3rd day (P < 0.01), gradually decreased on the 5th and 7th day (P < 0.01), and the level of TSP2 increased significantly with the duration of onset (P < 0.01).The brain edema volume in observation group on the 3rd, 5th and 7th days were significantly higher than that on the first day (P < 0.01), reached a peak on the 3rd day (P < 0.01), and gradually decreased on the 5th and 7th days (P < 0.01).On the 1st day of onset, the brain hematoma volume reached a peak (P < 0.01), and then gradually decreased (P < 0.01).The results of correlation analysis showed that the serum levels of TSP1, MMP2 and MMP9 were significantly positively correlated with brain edema volume (P < 0.01), and negatively correlated with the cerebral hematoma volume (P < 0.01).The serum level of TSP2 was significantly negatively correlated with brain edema volume (P < 0.01), and positively correlated with the cerebral hematoma volume (P < 0.01).

ConclusionThe serum levels of MMP and TSP in acute phase of cerebral hemorrhage are related to the cerebral edema volume, and the TSP can affect the degree of cerebral hematoma and edema by regulating the expression level of MMP.