Research of nuclear factor-κB oligodeoxynucleotide targeting strategy on cytokine regulation in collagen induced arthritic rats model

Objective: To investigate the therapeutic effect of nuclear factor-κB(NF-κB) oligodeoxynucleotide(ODN) Decoy topical application on typeⅡcollagen induced arthritis(CIA) rats and analyze its mechanism.Methods: Type Ⅱ CIA rats was established,NF-κB ODN Decoy was injected into the posterior ankle cavity of rats.The rats were divided into control group,CIA model group and experimental group.Forty-two days after the initial immunization,the arthritis scores were determined,the ankle pathology examination were performed,the interleukin(IL)-17,IL-12 and transforming growth factor(TGF)-β levels in serum and joint fluid were determined using by enzyme linked immunosorbent assay method.Results: Compared with control rats,IL-17,IL-12 levels in serum and joint fluid in model CIA rats were increased significantly,and TGF-β level was decreased(P<0.01).After the CIA rats were received with NF-κB ODN Decoy,compared with CIA rats,IL-17,IL-12 levels in serum and joint fluid were decreased significantly,and TGF-β level was increased significantly(P<0.01).Conclusions: NF-κB decoy had significantly inhibited the production of IL- 17,IL–12 in serum and joint fluid and promoted the increase of TGF-β,and it had a good curative effect on treatment of rheumatoid arthritis.