Validation of the microbial limit inspection method of guihongqufeng wine

Objective:To establish the method of the microbial limit inspection of guihongqufeng wine.Methods:The flocculent precipitation of preparation was removed by membrane-filter procedure.The microbial limit of sample was inspected by culture medium dilution and membrane-filter procedure.The methodology was validated by the results of 6 positive bacteria experimental group,microbial group and control group.Results:The recoverys of bacterial colony in Escherichia coli,Staphylococcus aureus,Bacillus subtilis,Candida albicans and Aspergillus niger were more than 70%.All positive bacteria could be detected by the control germ test.Conclusions:The membrane-filter procedure can count the bacterial colony,the test organisms can be checked out by the methods of liquid preparation and control germ test.