Effect of LP17 on the ultrastructure of intestinal macrophages of rats

Objective:To investigate the effects of LP17 on the ultrastructure of intestinal macrophages of rats in vitro, explore the activated mechanism of intestinal macrophage and search for the treatment of intestinal mucosal barrier dysfunction.Methods:The intestinal macrophages of rat were separated and cultured in vitro, and divided into the control group, (non-LPS and -LP17) and experiment group, which included the lipopolysacharide(LPS) group(treated with 1 mg/L of LPS) and LPS+LP17 group(treated with 1 mg/L of LPS +0.1 mg/L of LP17).Cells were digested by 0.25% trypsin, and collected after 6 h of incubation.The ultrastructure of macrophages in all groups were observed using Tecnai 12 transmission electron microscopy.Results:The normal macrophages in control group, a large number of lysosomes in macrophages of LPS group and apoptotic bodies in macrophages of LPS+LP17 group were found under electron microscopy after drug treatment.Conclusions:LP17 can induce the apoptosis in activated macrophages.