Effect of Sinomenine on the TLR4/MyD88 signal pathway in rats with adjuvant arthritis

Objective:To investigate the effects of Sinomenine on TLR4/MyD88 signal pathway in rats with adjuvant arthritis.Methods:Forty SD rats were randomly divided into the model group, Sinomenine group, methotrexate group and normal control group.The rat model of adjuvant arthritis in first three groups were induced by intradermal injecting Freund's into right paw, the normal control group were injected with saline.The mRNA expressions of TLR4 and MyD88 and level of TNF-α in knee synovium of four groups were detected by real-time PCR and ELISA after 4 weeks of intervention, respectively.Results:The levels of TLR4 and MyD88 mRNA expressions, and TNF-α of synovial tissue in model rats were significantly higher than those in normal control group(P<0.01).Compared with the model rats, the levels of TLR4 and MyD88 mRNA expressions, and TNF-α significantly were decreased after treatment with Sinomenine(P<0.01).Conclusions:Sinomenine can improve the adjuvant arthritis in rats, which may be by inhibiting the TLR4/MyD88 signal pathway.