The expression of breast cancer metastasis suppressor 1 in nasopharyngeal carcinoma cell lines

Objective:To detect the expressions of breast cancer metastasis suppressor 1(BRMS1) gene in different human nasopharyngeal carcinoma(NPC) cell lines.Methods:The expressions of BRMS1 protein and mRNA in SUNE-1-5-8F, CNE-1, CNE-2Z, SUNE-1 and SUNE-1-6-10B of NPC cell lines were detected by immunohistochemical method and RT-PCR, respectively.Results:The positive expressions of BRMS1 protein in CNE-1, CNE-2Z, SUNE-1, SUNE-1-5-8F and SUNE-1-6-10B cell lines were identified.Except the SUNE-1-5-8F cell line, the mRNA expressions of BRMS1 in other four NPC cell lines were positive.The expression levels of BRMS1 mRNA in CNE-1, CNE-2Zand SUNE-1 cell lines were significantly higher than that in SUNE-1-6-10B cell line.Conclusions:The expression of BRMS1 is correlated to the metastatic ability of cell line, which may be an important marker in identifying the metastasis of NPC.