Change of T lymphocyte subsets in patients with severe craniocerebral injury

Objective: To detect the change rules of T lymphocyte subsets in patients with severe craniocerebral injury to provide the basis in preventing infection by immunologic intervention.Methods: Thirty severe craniocerebral injury patients with glasgow coma score(GCS)≤8 and 30 light craniocerebral injury patients GCS =14 to 15 were divided into the observation group and control group,respectively.The expressions of lymphocyte subsets(including CD3,CD4,CD8,CD4/CD8 T) of peripheral blood in two groups after 1,3,7 d and 14 d of injury were detected using the immunofluorescent three-color flow cytometry.Results: Compared with the control group,the levels of CD3,CD4 and CD4/CD8 began to decrease,and the level of CD8 increased 1 d after injury in observation group.Compared with the control group,the levels of CD3,CD4 and CD4/CD8 decreased significantly,and the level of CD8 increased significantly 3 d after injury in observational group.The levels of lymphocyte subsets began to recover after 7 d of injury,and arrived at the normal level after 14 d of injury.The differences of the levels of lymphocyte subsets between two groups after 1 d,3 d and 7 d after injury were statistically significant(P<0.05 to P<0.01),and there was not statistical significance 14 d after injury(P>0.05).The lung infection rates in the observation group and control group after 4 d of injury were 73.3% and 0,respectively,the difference of which was statistically significant(P<0.01).Conclusions: Decreasing on day 1,significant decreasing on day 3,recovering on day 7 and arriving at the normal level on day 14 are the change rules of T lymphocyte subsets in severe craniocerebral injury patients.The immunologic intervention can be used to prevent infection in severe craniocerebral injury patients.