Effect of probucol combined with celecoxib on atherosclerosis in rats

Objective:To investigate the effects of probucol combined with celecoxib on atherosclerosis in rats.Methods:Forty male SD rats were randomly divided into the normal group, model group, probucol group, celecoxib group and probucol combined with celecoxib group(8 rats each group).Normal group was fed with standard forage.Rat model with atherosclerosis was established, and treated with high lipid, Vitamin D3 plus balloon injury of endarterium.The probucol group, celecoxib group and probucol combined with celecoxib group were treated with oral gavage, respectively.After 12 weeks of drug intervention in all groups, the serum levels of total cholesterol(TC), triglyceride(TG), low density lipoprotein-cholesterol(LDL-C) and high density lipoprotein-cholesterol(HDL-C) were detected, the levels of serum C-reactive protein(CRP) and Pentraxin3(PTX3) were determined by ELISA, and the pathomorphological changes of thoracic aorta were measured using HE staining and the thickness of arterial intima and media and thickness ratio of intima to media were calculated.Results:Compared with the normal group, the serum levels of TC, LDL-C and CRP in model group, probucol group, celecoxib group and probucol combined with celecoxib group significantly increased(P<0.01).Compared with the model group, the serum levels of TC, LDL-C, CRP and PTX3 in probucol group and probucol combined with celecoxib group significantly decreased(P<0.01).Compared with the normal group, the thickness of arterial intima and media, and thickness ratio of intima to media in probucol group, celecoxib group and probucol combined with celecoxib group significantly increased(P<0.01).Compared with the model group, the thickness of arterial intima and thickness ratio of intima to media significantly decreased and the thickness of arterial media significantly increased in probucol group, celecoxib group and probucol combined with celecoxib group(P<0.01).Conclusions:The combination use of probucol and celecoxib can significantly decreased the levels of serum lipids and inflammatory factors in rats with atherosclerosis, which has certain effects on anti-atherosclerosis.