Effect of Artemisiae capillaries on the expressions of hepatic membrane protein Mrp3 and nuclear receptor Pxr in rats

Objective:To observe the effects of Artemisiae capillaris on the expressions of multidrug resistance-associated protein 3(MRP3) and nuclear pregnane X receptor(PXR) in normal rat liver.Methods:Ten male Sprague-Dawley(SD) rats were randomly divided into the Artemisiae capillaris group and control group(5 rats each group).The Artemisiae capillaris group and control group were treated with gavage of Artemisiae capillaris(100 mg·kg-1·d-1) and gavage of 0.9% sodium chloride.Two groups were sacrificed after 7 days.The morphological change of liver tissue was observed using HE staining,the total RNA and membrane or nuclear protein were extracted from the liver tissue of two groups.The mRNA and protein expressions of MRP3 and PXR were detected by Real-time PCR and Western blotting,respectively.Results:Compared with the control group,the morphological structure of liver tissue in Artemisiae capillaris group was not influenced.Artemisiae capillaris could significantly up-regulate the protein expressions of MRP3 and PXR in rat liver(PConclusions:Artemisiae capillaris can up-regulate the expression of MRP3 and PXR in rat liver.