The effects of sinomenine combined with 5-fluomuracil on the proliferation and apoptosis in human gastric gland carcinoma cell line SGC 7901

Objective:To explore the effects of sinomenine combined with 5-fluomuracil(5-Fu) on the proliferation of human gastric gland carcinoma cell line SGC 7901 and expressions of Bcl-2,Bax and COX-2.Methods:The human gastric gland carcinoma cell line SGC 7901 was cultured in vitro,which was divided into the control group,5-Fu group(50 mg/L),low concentration sinomenine group(1.25 mmol/L),medium concentration sinomenine group(2.5 mmol/L),high concentration sinomenine group(5 mmol/L) and combination group(25 mg/L of 5-Fu and 2.5 mmol/L of sinomenine).The proliferation of SGC7901 cells was detected by MTT,the cell cycle and apoptosis rate were determined by flow cytometry,and the expressions of Bcl-2,Bax and COX-2 were detected by immunohistochemistry.Results:Sinomenine could inhibit the proliferation of SGC7901 cells after 24 h,48 h and 72 h of treatment,which was dose- and time-dependent,and the treatment with sinomenine combined with 5-Fu could strengthen the inhibitory effects(P<0.05 to P<0.01).Compared with the control group,the apoptosis rates in 5-Fu group,sinomenine groups and combination group increased obviously(P<0.01),and the apoptosis rate in combination group was significantly lower than that in single drug group(P<0.05 to P<0.01).The positive expressions of Bcl-2 and COX-2 in the control group,and the sinomenine group,5-Fu group and combination group were strong and weak,respectively,the differences of whose were statistical significance(P<0.05 to P<0.01).The positive rates of Bcl-2 and COX-2 expressions in combination group were lower than those in single drug group(P<0.01 ).The positive expression of Bax in the control group,and the sinomenine group,5-Fu group and combination group were weak and strong,respectively,the differences of whose were statistically significant(P<0.01).The positive rate of Bax expression in combination group was higher than that in single drug group(P<0.01).Conclusions:Sinomenine combined with 5-Fu can enhance the inhibitory effect on SGC7901 cells proliferation,which can further upregulate the expression of Bax,and downregulate the expressions of Bcl-2 and COX-2 protein.