The detection of Anti-HCV,HCV-RNA and its genotype in 5 080 patients with hepatitis C

Objective:To analyze the hepatitis C antibody(Anti-HCV),HCV-RNA and genotype in 5 080 patients with hepatitis C.Methods:The levels of Anti-HCV and HCV-RNA in all patients were determined by automatic enhanced luminescence immunity analyzer and quantitative fluorescent PCR,respectively.The genotypes in the patients with more than or equal to 10³ of HCV-RNA were detected.Results:The positive rates of Anti-HCV and HCV-RNA were 99.8% and 49.1%,respectively.The ages and antibody content in positive HCV-RNA patients were significantly higher than those in the negative HCV-RNA patients(P<0.01).Among the 1 723 positive HCV-RNA specimens,the 1b and 2a genotypes accounted for 59.49% and 36.20%,respectively,the total ratio of 1b and 2a genotypes was 95.69%.The unusual types of 3b and 4,and mixed infection types of 1b/2a and 1b/4 also were detected.Conclusions:The Anti-HCV content and viral load are lack of correlation,which can not be used as the observation indexes in estimating the treatment.The quantitation of HCV-RNA can be used to evaluate the curative effect and prognosis of patients with hepatitis C.The 1b and 2a are the major genotypes in patients with hepatitis C.To analyze the genotype of the positive HCV-RNA patients can provide the basis in personalized therapy.