Effect of γ-interferon on the injury degree of liver in suckling mouse with rotavirus infection

Objective: To explore the expression of hepatic NF-κB for evaluating the effects of IFN-γ on suckling mouse with rotavirus(RV) infection.Methods: The suckling mouse were divided into the control group,high and low-dose IFN-γ groups.The extraintestinal rotavirus infection model of 2-day-old suckling mouse was established by intraperitoneal injection of rotavirus.The 2μg and 0.2μg IFN-γ were injected subcutaneously into the back of BALB/C suckling mouse for 3 days.The pathological changes and expressions of NF-κB detected by Western blot and immunohistochemistry in liver were observed after 5 days of model establishment.Results: The difference of the score of liver pathological damage between low-dose IFN-γ group and control group was not statistically significant(P>0.05).The score of liver pathological damage in high-dose IFN-γ group was significantly higher than that in control group and low-dose IFN-γ group(P<0.01).Western blotting and immunohistochemistry results showed that the expression of NF-κB increased with the dose increasing of IFN-γ(P<0.01).Conclusions: IFN-γ can stimulate the expression of NF-κB.The pathological damage of the liver was correlated with the expression of NF-κB.