The immune response of T cell to HBV antigen protein in different types HBV infecting

Objective:To explore the T cell immune response function in different clinical manifestation patients.Methods:The peripheral blood mononuclear cells were isolated.The T cell subgroup was detected using flow cytometry,and the immune response of T cell to HBV antigen protein was detected using enzyme-linked immunespots assay.Results:Compared with the acute hepatitis patients(group A),the frequency of CD4⁺ T cell subgroup in chronic hepatitis patients(group B) and asymptomatic carriers(group C) were significantly lower(P<0.01),and the differences of the frequency of CD8⁺ T cell subgroup in 3 groups were not statistically significant(P>0.05).Compared with group A,the ratios of CD4⁺/CD8⁺ in group B and group C were decreased(P<0.01).The spof forming call in group A was significantly less than that in group B and group C(P<0.01),and the spof forming call in group B was significantly less than that in group C(P<0.01).Conclusions:T cell immune in patients with different clinical manifestations is different,and the dysequilibrium of T cell subgroup may lead to immune disorders,and can not clear the virus,which is the cause of different clinical symptoms.