Comparision of the different stimulants on the peripheral blood T cells of healthy people and tuberculosis patients producing interferon-γ and tumor necrosis factor-α

Objective:To detect the changes of the different stimulants on the peripheral blood T cell subsets of healthy people and tuberculosis(TB) patients producing interferon-γ(IFN-γ) and tumor necrosis factor-α(TNF-α).Methods:The proportion of T cell subsets producing INF-γ and TNF-α in 12 healthy people peripheral blood mononuclear cells and 12 TB patients peripheral blood stimulated with PMA combined with ionomycin, Mtb-Hag, PHA and blocked with monensin were detected using the flow cytometry.Results:The proportion of INF-γ produced by αβ T cells of peripheral blood stimulated with PMA in healthy people was significantly higher than that in TB patients(P<0.05).The proportions of INF-γ produced by γδ T cells of peripheral blood stimulated with PMA, Mtb-Hag and PHA in healthy people were higher than that in TB patients(P<0.05 to P<0.01).The proportions of TNF-α produced by αβ T cells of peripheral blood stimulated with PMA PHA in healthy people were higher than those in TB patients(P<0.05).The proportions of INF-α produced by γδ T cells of peripheral blood stimulated with PMA and PHA in healthy people were higher than that in TB patients(P<0.05).Conclusions:After stimulated with different stimulants, the proportion of INF-γ produced by γδ T cells in healthy people is higher than that in TB patients.After simulated with PMA and PHA, the proportion of TNF-α produced by αβ T cells and γδ T cells in healthy people is higher than that in TB patients.