The protective effect of carbachol combined with dietary fiber on the intestinal barrier in rats with burn shock

Objective:To explore the protective effects of carbachol combined with dietary fiber on the intestinal barrier in rats with burn shock.Methods:The animal model of 80 Wistar rats with 30% TBSA Ⅲ° burn were established,and randomly divided into the saline injection group(group A),dietary fiber treatment group(B group),carlbachol treatment group(groupC) and carlbachol combined with dietary fiber treatment group(group D)(20 rats each group).The 4 rats in each group were sacrificed at 6,12,24,48 and 72 hours after injury,respetcively.The 4 normal rats were set as the control group(group N).The pathomorphological change of small intestine was observed under light microscope,the plasma levels of endotoxin in portal vein and D-lactic acid in abdominal aorta were detected.Results:The damage of the intestinal tissue in each burn group was significant.The plasma levels of endotoxin and D-lactic acid in all burn rats were significantly higher than those in group N,which arrived at peak at 12 to 24 hours after injury,and did not recover until 72 hours after injury.The treatment of the carlbachol combined with dietary fiber of single carlbachol or dietary fiber could significantly decrease the levels of the plasma endotoxin and D-lactic acid(P<0.05 to P<0.01).Conclusions:The early application of carlbachol combined with dietary fiber has markedly protective effect on intestinal barrier in burn rats,the treatment effect of which is better than that in single treatment of carbachol or dietary fiber to some exent.