Protective effects of soybean isoflavones combined with fasudil on cerebral ischemia reperfusion injury in rats

Objective: To investigate the effects of soybean isoflavones(SI) combined with fasudil(Fas) on the expressions of high mobility group box 1(HMGB1),vascular cell adhesion molecule-1(VCAM-1),nuclear factor κB1(NF-κB1) in rats with cerebral ischemia reperfusion.Methods: The weight 240 to 260 g male SD rats were randomly divided into the sham group(S group),ischemia reperfusion(IR group),IR and Fas group,IR and SI group,and IR,Fas and SI group.The cerebral pathological change was observed by HE staining.The qualitative and quantitative expressions of NF-κB1(p50) and HMGB1 in cerebral tissue were measured by immunohistochemistry and western blotting,respectively.The expressions of HMGB1,NF-κB1 and VCAM-1 mRNA were examined by qRT-PCR.Results: Compared with the single drug group,the decreasing of the positive cells with NF-κB1 and HMGB1 expressions in IR,Fas and SI group were the more significant.Compared with the S group,the protein expression of HMGB1,and mRNA expression of HMGB1,NF-κB1and VCAM-1 in IR group increased significantly(P < 0.01).Compared with the IR group,the protein expression of HMGB1,and mRNA expressions of HMGB1,NF-κB1and VCAM-1 in other drug groups decreased significantly(P < 0.01).Compared with the single drug group,the decreasing of the protein expression of HMGB1,and mRNA expressions of HMGB1,NF-κB1 and VCAM-1 in IR,Fas and SI group were the more significant(P < 0.01).Conclusions: The protective effects of SI combined with Fas on cerebral ischemia reperfusion injury in rats maybe be implemented by inhibiting the expressions of HMGB1,NF-κB1 and VCAM-1,which can attenuate the signal channel of NF-κB,and decrease the cerebral tissue injury casued by inflammatory response.The protective effects of drug combination is significantly better than that of single drug.