Identification capability and application value of automatic microorganism identification drug sensitivity analyzer for clinically relevant bacteria

Objective: To investigate the identification capability and application value of VITEK 2 system automatic microorganism identification drug sensitivity analyzer for clinical bacteria.Methods: One hundred and twenty-six strains of pathogenic bacteria isolated from respiratory tract,urogenital tract,pus and blood sample were selected,and 60 reference strains were served as control.The drug sensitivity of pathogenic bacteria was identified using VITEK 2 system automatic microorganism identification drug sensitivity analyzer,the correct rate,identification time and drug sensitivity of whose were analyzed.Results: The four kinds of germplasm control strains was tested for 10 times,a total of 40 times,the repetition rate of identification was 100%.Among 90 strains of gram negative bacteria,87 strains were identified,and the correct identification rate of which was 96.67%.Among 96 strains of gram positive bacteria,93 strains were identified,and the correct identification rate of which was 96.88%.The identifications of enterobacter sp,non fermentation bacteria,streptococcus sp.and micrococcus were completed within 9 h,the 85% identification of enterobacter sp.and micrococcus were completed within 6h.The coincidence rate of drug susceptibility in gram positive bacteria and gram negative bacteria were 93.94% and 93.40%,respectively,and the total coincidence rate was 93.66%.Conclusions: The correct identification rate of VITEK 2 automatic microorganism identification drug sensitivity analyzer is high,which can quickly identify and guide the rational use of antimicrobial drugs,and has important clinical value in disease diagnosis.