Effect of cypermethrin on the oxidative damage and mRNA expression of nuclear factor erythroid-2-related factor 2 in mice cerebellar tissue

Objective: To observe the effects of cypermethrin on the oxidative damage and gene expression of nuclear factor erythroid-2-related factor 2(Nrf 2) mRNA in mice cerebellar tissue.Methods: Forty male Kunming mice were randomly divided into one negative control group and three cypermethrin infection groups.Three cypermethrin infection groups were infected by 5,10 and 20 mg/kg of cypermethrin gavage,once daily for three weeks,respectively.The contents of MDA,GSH-Px,T-SOD and CAT and MDA were measured in cerebellar tissue.The mRNA and protein expressions of Nrf2 in cerebellar tissue were detected using reverse transcription polymerase chain reaction(RT-PCR) and immunohistochemistry(IHC),respectively.Results: With the dose of cypermethrin increasing,the level of MDA increased,the levels of GSH-Px,T-SOD and CAT decreased in cerebellar tissue,and the differences of whose between the cypermethrin infection group and control group were statistically significant(P<0.01).The immunohistochemistry result showed that the positive expression of Nrf2 in nucleus increased in cerebellar tissue with the dose of cypermethrin increasing,the difference of which between the cypermethrin infection group and control group were statistically significant(P<0.01).The positive expression of Nrf2 in nucleus in high-dose cypermethrin infection group was higher than that in low-dose cypermethrin infection group(P<0.01).The RT-PCR results showed that the optical density value of Nrf2 increased in cerebellar tissue with the dose of cypermethrin increasing,the difference of which between the cypermethrin infection group and control group were statistically significant(P<0.01).The mRNA expression level of Nrf2 in high-dose cypermethrin infection group was higher than that in low-dose cypermethrin infection group(P<0.01).Conclusions: The cypermethrin infection can lead to the oxidative damage in cerebellar tissue and induce the translocation of Nrf2 from the cytoplasm to the nucleus,which can strength the antioxidative capacity of brain tissue.