The feasibility study of acellular dermal matrix reconstructed with autologous platelet-rich plasma combined with bone marrow stem cells in the repair of cartilage of rabbit

Objective: To investigate the effects and clinical value of acellular dermal matrix(ADM) reconstructed with autologous platelet-rich plasma(PRP) combined with bone marrow stem cells(BMSCs) in the repair of cartilage of rabbit.Methods: Sixty rabbits with femoral condylar articular injury were randomly divided into the study group,control group and blank group(20 rabbits each group).The PRP and BMSCs were isolated,cultured,and inoculated with the ADM scaffolds in vitro.The 10% of PRP was added to the culture medium in study group,and the other treatment was the same as the control group.The blank group did not give any process.The BMSCs-ADM composite was implanted into the articular defect of rabbits.The reconstructing cartilage tissue was detected using the immune mechanics,histology and biomechanics at the end of 1,2 and 3 months of implantion.The restoration effects of joints defect with PRP-BMSCs-ADM were analyzed.Results: With the prolongation of the culture time,the number of BMSCs cells increased significantly,the growth curve of cells appeared S-shaped,which arrived at logarithmic phase and plateau phase after 3th day and 9th day,respectively.From the 3th day,the OD value of cell counts in study group was significantly higher than that in control group(P<0.01).With the extension of time,the number of cartilage-like cells in all groups increased,the cartilage space matured gradually,the surface of cartilage smoothed gradually,the macro scoring of IRCS and histological scoring increased significantly(P<0.01),the elastic modulus of the specimens pressure increased(P<0.05).During the same period,the specimen scoring and pressure elastic modulus in study group were significantly higher than those in control group and blank group(P<0.01).After 3 months,the repair of cartilage defect in study group was significantly better than that in control group and blank group(P<0.01).Conclusions: The effects of ADM reconstructed with PRP combined with BMSCs in repairing the cartilage of rabbit are good,which is feasible.