The application value of the combined detection of peptidylarginine deaminase-4 and anti-cyclic citrullinated peptide antibody in the diagnosis of rheumatoid arthritis

Objective: To investigate the value of the combined detection of peptidylarginine deaminase-4(PADI-4) and anti-cyclic citrullinated peptide(anti-CCP) antibody in the diagnosis of rheumatoid arthritis(RA).Methods: The levels of PADI-4,anti-CCP antibody,anti-MCV antibody,AFA antibody and anti-RA33 antibody in 58 patients with RA(RA group),40 patients with other rheumatoid disease(non-RA group) and 30 healthy people(control group) were detected by ELISA.The ROC curve,sensitivity,specificity,relevance and diagnostic effectiveness in all cases were analyzed.Results: The serum levels of PADI-4,anti-CCP antibody,anti-MCV antibody,AFA antibody and anti-RA33 antibody in RA patients were significantly higher than those in non-RA group and control group(P<0.01).The ROC curve showed that the best critical value of PADI-4 and anti-CCP antibody in the diagnosis of RA were 1.284 U/L and 26.365 RU/mL,the sensitivity and specificity of PADI-4 and anti-CCP antibody were 62.1% & 81.1% and 91.4% & 95.7%,respectively.PADI-4 was positive correlation with AFA antibody,anti-RA33 antibody and anti-MCV antibody(P<0.01),but the PADI-4 was not correlation with anti-CCP antibody(P>0.05).Among the combined detection of multiple antibodies,the specificity of the PADI-4 and anti-CCP antibody in the diagnosis of RA was 100.0%,and the positive detection of PADI-4 and anti-CCP antibody were 91.4% and 95.7%,respectively.Conclusions: The combined detection of PADI and anti-CCP antibody can improve the specificity and sensitivity of RA,which can help the diagnosis of RA.