Effect of hydrogen peroxide on the expression of in terleukin-3 in hurnan umbilical vein endothelial cells

Objective: To investigate the effects of hydrogen peroxide(H₂O₂) on the expression of interleukin-37(IL-37) in EA.hy926 cells.Methods: The expressions of IL-37 mRNA and protein in different time-points of human umbilical vein endothelial cell treated with different concentration of H₂O₂ were detected by qRT-PCR and Western blot.Results: The results of qPCR showed that the levels of IL-37 mRNA in human umbilical vein endothelial cell treated with different concentration of H₂O₂ significantly increased,which was the concentration-dependent(P<0.01).The results of Western blot showed that the level of IL-37 protein in human umbilical vein endothelial cell treated with H₂O₂ for 20 h was significantly higher than that in control group(P<0.01).Conclusions: The expression of IL-37 in human umbilical vein endothelial cell can be up-regulated by H₂O₂,which may be helpful to study the role of IL-37 in cellular oxidative stress.