Effect of recombinant human granulocyte colony stimulating factor on the expression of apoptosis inducing factor in neonatal rats with hypoxic-ischemic brain damage

Objective: To study the effects of recombinant human granulocyte colony stimulating factor(rhG-CSF) on the expression of apoptosis inducing factor(AIF) in neonatal SD rats.Methods: Thirty-six healthy neonatal SD rats with 7 days old were randomly divided into the sham operation group(group A),cerebral ischemia reperfusion group(group B) and drug treatment group(group C)(12 rats each group).The rat cerebral arterial occlusion reperfusion model was made using Longa suture blot method.The group C were injected with drug after two hours and 24 hours of cerebral ischemia,and the other two groups were injected with the same amount of normal saline.At the end of the experiment,the neural function of rat was evaluated by Longa 5 score method,the expressions of extracellular signal regulated protein kinase(p-ERK) and phosphorylated extracellular signal regulated protein kinase(p-JNK) were detected by immunohistochemical method,and the neuronal apoptosis and cerebral infarction volume were detected using Tunel and TTC staining,respectively.The levels of expression and apoptosis of apoptosis related genes were evaluated.Results: A small part of the apoptotic cells in A group and large number of apoptotic cells and brown nuclei in groups B and C after 24 h of reperfusion were found,which distributed mainly around the ischemic brain tissue,and which in C group was less than that in group B(P<0.01).Conclusions: rhG-CSF can protect the neuron of neonatal rat,and avoid the occurrence of ischemic hypoxic brain damage,which is related to the expression of the apoptosis related gene.