Effect of luteolin on the proliferation,differentiation, mineralization and Wnt pathway of MC3T3-E1 osteoblasts in vitro

Objective: To investigate the effects of luteolin on the proliferation,differentiation,mineralization and Wnt pathway of MC3T3-E1 osteoblasts in vitro.Methods: The MC3T3-E1 osteoblasts were cultured with the 0.25 to 8.00 μmol/L of luteolin for 12,24 and 48 h in vitro.The 0.04 μmol/L of lovastatin treating cell was used as the positive control group.The proliferation,activity of alkaline phosphatase(ALP) and mineralization capacity of MC3T3-E1 osteoblasts in vitro were detected by cell counting kits-8 method,alkaline phosphatase kit and Alizarin Red S staining,respectively.The mRNA expressions of type Ⅰ collagen,osteocalcin,low density lipoprotein receptor related protein 5(Lrp5),β-catenin,osteoprotegerin and nuclear factor κB-activating factor receptor ligands were quantified by real-time reverse transcription PCR(qPCR).Results: Luteolin could improve the proliferation of MC3T3-E1 osteoblasts with time- and dose-dependence(P<0.05 to P<0.01).Luteolin could promote the activity of ALP,strengthen the mineralization capacity and upregulate the mRNA expressions of type Ⅰ collagen,osteocalcin,Lrp5,β-catenin,osteoprotegerin and nuclear factor κB-activating factor receptor ligands in MC3T3-E1s with dose-dependence in vitro.Conclusions: The 2.00,4.00 and 8.00 μmol/L of luteolin can promote the proliferation,differentiation and mineralization and the expressions pf Lrp5 and β-catenin in MC3T3-E1 osteoblasts.