The distribution of immune subtpyes of αβ and γδT cell subsets of peripheral blood in patients with tuberculosis

Objective:To investigate the distribution of immune subtpyes of αβ and γδT cell subsets of peripheral blood in healthy adults and pulmonary tuberculosis(TB) patients.Methods:The peripheral blood from healthy adults and TB patients were stained with different fluorochrome-conjugated mAbs.The CD45RA⁺CD27⁺ naive T cells(Tnaive),CD45RA^-CD27⁺ central memory T cells(Tcm),CD45RA^-CD27^- effector memory T cells(Tem) and CD45RA⁺CD27^- effector T cells of αβ T cells immune subtpyes,and the proportions of the Vδ1,Vδ2,Vδ1^-Vδ2^-(Vδ3-8) immune subtypes T cells in γδ T cells were detected using flow cytometry.Results:Compared with healthy adults,the percentages of Tnaive & Teff cells,and Tem cells in CD4⁺ αβ T cells of TB patients decreased and increased,respectively(P<0.01),and the difference of Tcm cell between healthy adults and TB patients was not statistically significant(P>0.05).Compared with healthy adults,the percentages of Tnaive & Tcm cells,and Tem & Teff cells decreased and increased in CD8⁺ αβ T cells of TB patients,respectively(P < 0.01 and P < 0.05).Compared with healthy adults,the percentages of Tnaive & Tcm cells,and Tem cells decreased and increased in CD8⁺ αβ T cells of TB patients,respectively(P<0.05),and the difference of Teff cells between healthy adults and TB patients was not statistically significant(P>0.05).The percentages of Tcm cells,and Tem & Teff cells in Vδ2 γδ T cells of TB patients decreased and increased,respectively(P < 0.05 and P < 0.01),and the difference of Tnaive cells between healthy adults and TB patients was not statistically significant(P>0.05).The percentage of Tem cells in Vδ3-8 γδ T cells of tuberculosis patients increased(P<0.05),and the differences of the percentages of Tnaive,Tcm and Teff cells between healthy adults and TB patients were not statistically significant(P>0.05).Conclusions:The changes of the αβ T and γδ T cell subsets distributions in peripheral blood of TB patients are significant.