Study on the correlation of complement C5a with IL-6 in CLP septicemic mice

Objective:To explore the relationship between inflammatory mediator C5a and interleukin-6(IL-6) in the pathogenesis of sepsis.Methods:The animal model of sepsis was established using the cecal ligation and puncture(CLP).During the active stage of sepsis,the expressions of completement C5a and IL-6 in peritoneal lavage fluid of the different time-points model group and sham operation group were detected.Results:The levels of the complement C5a and IL-6 in peritoneal lavage fluid in model group after 4,6,12 and 24 h of establishing model were significantly higher than those in sham group(P<0.01).With the recovery of disease,the levels of complement C5a and IL-6 in two groups gradually returned to the normal level.Conclusions:During the pathogenesis of CLP sepsis in mice,the inflammatory mediators C5a and IL-6 have a linear correlation trend.