Effect of diammonium glycyrrhizinate on peritoneal connective tissue growth factor in retroperitoneal fibrosis rats treated with peritoneal dialysis

Objective: To investigate the effects of diammonium glycyrrhizinate on peritoneal connective tissue growth factor(CTGF) in retroperitoneal fibrosis rats treated with peritoneal dialysis.Methods: Fifty male SD rats were randomly divided into the control group,NS group,model group,pentoxifylline group and diammonium glycyrrhizinate group(10 rats each group).The peritoneal dialysis model of rats was established.The pentoxifylline group and diammonium glycyrrhizinate group were treated with pentoxifylline and diammonium glycyrrhizinate for 28 days,respectively.On the 30th day,the 4 h peritoneal equilibration test was adopted to determine the rats' peritoneum function,the partial peritoneum tissue of rats was collected to observe the pathological morphology and thickness,and the expression of CTGF in peritoneal tissue was detected.Results: The HE staining showed that a layer of flattened cells covered the peritoneum surface were found in control group and NS group,and the column and round cells,abstraction,matrix thickening,a large number of fibroblast-like cells,monocytes and macrophages infiltration,and fibrinoid material deposition in peritoneal mesothelial cells were found in model group,pentoxifylline group and diammonium glycyrrhizinate group.Masson staining showed that the apparent collagen deposition was found,which in model group was more than that in pentoxifylline and diammonium glycyrrhizinate group.The peritoneum thickness in model group,pentoxifylline treatment group,diammonium glycyrrhizinate treatment group,control group and NS group gradually decreased(P<0.01).The peritoneal ultrafiltration and D4/D0 in control group and NS group pentoxifylline group,diammonium glycyrrhizinate group and model group gradually decreased(P<0.01).The value of Durea/Purea and CTGF level of peritoneum in model group,pentoxifylline group,diammonium glycyrrhizinate group,control group and NS group gradually decreased(P<0.01).Conclusions: The 4.25% peritoneal dialysis solution can lead to peritoneal fibrosis in rats,and the diammonium glycyrrhizinate can alleviate peritoneal the peritoneal fibrosis and function.