Clinical application of combined detection of tumor markers in the diagnosis of breast cancer

Objective:To investigate the clinical value of combined detection of the serum cancer antigen15-3(CA15-3),carcinoembryonic antigen(CEA),tumor specific growth factor(TSGF),cancer antigen125(CA125) and growth differentiation factor 3(GDF3) in the diagnosis of breast cancer.Methods:Fifty-six patients with primary breast cancer,44 patients with benign breast tumor and 52 healthy females were divided into the breast cancer group,benign group and control group,respectively.The 3ml peripheral blood of fasting state in all cases was collected,and centrifuged.The serum levels of CA15-3,CA125 and CEA were detected by chemiluminescence method,the TSGF was detected by chemical colorimetric method,and GDF3 was detected by enzyme linked immunosorbent assay.Results:The serum levels of five tumor markers in breast cancer group were significantly higher than those in benign group and control group(P<0.01).Except for the level of TSGF in benign group were significantly higher than that in control group(P<0.01),and the differences of the levels of other four markers between benign group and control group were not statistically significant(P>0.05).The sensitivities of the separate detection of CA15-3,CEA,TSGF,CA125 and GDF3 were 32.1%,26.8%,21.4%,17.9% and 16.1%,respectively,and the sensitivity of combined detection was 64.3%,and higher than that of one tumor marker detection(P<0.05).The specificities of the separate detection of CA15-3,CEA,TSGF,CA125 and GDF3 were 88.6%,97.7%,77.3%,68.2% and 77.3%,respectively,and the specificity of combined detection was 72.7%.The specificity of CEA detection was the best,and which was higher than that of GdF3,CA125 and TSGF detection alone(P<0.05).The accuracies of the separate detection CA15-3,CEA,TSGF,CA125 and GDF3 were 57.0%,58.0%,46.0%,40.0% and 43.0%,respectively.The accuracy of combined detection was 68.0%,and which was higher than that of GDF3,CA125 and TSGF detection alone(P<0.05).Conclusions:The combined detection of tumor markers in the diagnosis of breast cancer has more higher sensitivity and accuracy,which can reduce the clinical misdiagnosis rate,and has important clinical application value.