Effect of 3-bromopyruvate on the proliferation and apoptosis of cisplatin-resistance nasopharyngeal carcinoma cells

Objective:To investigate the effects of glycolytic inhibitor 3-bromopyruvate(3-BP) on the proliferation and apoptosis in cisplatin-resistance nasopharyngeal carcinoma cells.Methods:The proliferation-inhibitory effect of 3-BP on HNE1/DDP cells was measured by MTT assay and colony formation assay,and the effect of 3-BP on the apoptosis of HNE1/DDP cells was detected by PI staining.The expression levels of poly ADP-ribose polymerase(PARP,apoptosis-related protein),myeloid cell leukemia-1(Mcl-1),B cell lymphoma/leukemia-2(Bcl-2) were analyzed using Western blotting.Results:3-BP could significantly inhibit the proliferation of HNE1/DDP cells,the IC₅₀ value of 48 h was 260.2 μmol/L,and the low concentrations(10,20,40 μmol/L) 3-BP could obviously inhibit the colony formation of HNE1/DDP cells.The 3-BP could significantly induce the apoptosis of HNE1/DDP cells,and the apoptosis rates of cells treated with 80,160,and 320 μmol/L of 3-BP for 48 h were (13.7±2.1)%,(25.5±2.4)% and(45.5±3.5)%,respectively,and the apoptosis rate of cells in control group was(1.6±0.6)%.The results of Western blotting indicated that the HNE1/DDP cells treated with 3-BP could promote the cleavage of PARP,and downregulate the expression levels of the antiapoptotic proteins Mcl-1 and Bcl-2.Conclusions:3-BP can inhibit the proliferation and induce the apoptosis of HNE1/DDP cells,the mechanism of which may be correlated with the downregulation of antiapoptotic proteins Mcl-1 and Bcl-2.