Cleavage effect of type 10-23 deoxyribozyme on HBV S and C gene in vitro
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Abstract
Objective:To explore the specific cleavage activities of type 10-23 deoxyribozyme 10-23 DRz enzymes on HBV S and C RNA in vitro.Methods:The recombinant plasmids containing HBV S and C gene were constructed,respectively.The HBV S or C gene RNA was synthesized in vitro using the linearized recombinant plasmid as template.The DRz-hbvs-1 and DRz-hbvc-1 targeting to cleave HBV S and C mRNA were designed and synthesized,respectively,and the cleavage activities of which on targeting RNA were observed in vitro.Results:The DRz-hbvs-1 and DRz-hbvc-1 could specifically and effectively cleave the targeting RNA,and the cleavage of which in nonDRz and ASODN control groups were not found.Conclusions:The 10-23 DRz can specifically cleave the HBV S and C gene RNA in vitro.
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