The expression level of miRNA-101 in gastric cancer tissue and cell,and its influence on biological behavior of gastric cancer

Objective:To explore the expression levels of miRNA-101 in gastric cancer tissue and cell,and its influence on biological behavior of gastric cancer cells.Methods:The expression levels of miRNA-101 in 31 gastric carcinoma tissue and paraneoplastic tissue samples,human gastric carcinoma cell line SGC-7901 and MKN45,and normal human gastric epithelial cell line GES1 were detected using real-time PCR method.The recombinant adenovirus vector containing miRNA-101 was established,and transfected into the gastric cancer cell line SGC-7901 and MKN45.The effect of miRNA-101 on gastric cancer cell proliferation was detected by MTT method,and the migration and invasion of gastric cancer cells were detected by Transwell method.Results:The expression level of miRNA-101 in gastric cancer tissue was significantly lower than that in adjacent tissue(P<0.01).The expression levels of miRNA-101 in SGC-7901 and MKN45 cell lines were significantly lower than that in GES1 cells(P<0.01).The proliferation of MKN45 cells in miRNA-101 group after 48 h of incubation was significantly lower than that in control group(P<0.05).The proliferations of SGC-7901 and MKN45 cells in miRNA-101 group after 72 h of incubation were lower than that in control group(P<0.05).The migration test results showed that the average migrated number of SGC-7901 and MKN45 cells in miRNA-101 group were significantly lower than that in control group(P<0.01).The invasion test results showed that the average invaded number of SGC-7901 and MKN45 cells in miRNA-101 group were significantly lower than that in control group(P<0.01).Conclusions:The expression levels of miRNA-101 in gastric cancer tissue and gastric cancer cell lines downregulate,the transfection of miRNA-101 can significantly decrease the abilities of proliferation,migration and invasion of SGC-7901 and MKN45 cells.The miRNA-101 may be involved in the occurrence and development of gastric diseases.